5. When using CPT in the context of a multisite study, we have seen that gel plugs can loosen and the cell separation can fail when tubes become too cold during shipping. By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. 5.3 Submitted by: Becton, Dickinson and Company 1 Becton Drive . Ruitenberg et al., 2006 This tutorial video describes the blood collection with aspiration technique and the safet. government site. Evacuated tube intended for the collection of whole blood and the separation of mononuclear cells. Similarly, when multiple sites are involved, it is critical to ensure success that the correct centrifugation G force is calculated and implemented across sites with potentially different instrumentation. Inpatient collection supplies are obtained through material services. Citrate; Blue tops & ACD; Yellow tops) should always be filled to their full draw volume for chemical analysis; this is. If shipping to another location, wrap in protective foam and ship via same-day or overnight courier to the processing lab at room temperature. We will not share your information for any other purposes. Ruitenberg J. J., Mulder C. B., Maino V. C., Landay A. L. and Ghanekar S. For this reason, an insulated shipping container is highly recommended, especially in cold winter climates. Bethesda, MD 20894, Web Policies Description. Privacy Policy. 30382903678748. Log In to Order View Alternatives Compare Product 207068. The majority of BD Vacutainer® Blood Collection Tubes are made of clear latex-free Please use the form below to provide feedback related to the content on this product. 8600 Rockville Pike **NOTE: Our testing is validated for BD vacutainers. Sample container Tube dimensions Ø x L (mm) 13 x 75 13 x 100 16 x 100 16 x 125 24 x 90 . ); this reduces variability in PBMC isolation between technicians. Log in for pricing and availability. Please use the form below to provide feedback related to the content on this product. Reduce the need to retest, relabel, and/or recollect specimens. Mix the blood by gentle inversion several times to ensure a homogeneous suspension. 9:00am-1:00pm. Blood collection with the SARSTEDT S-Monovette® (ISO colour code). Place the cold pack and sample tube in a plastic bag, Fill all dead space with crumpled paper or bubble wrap, Complete an AHDC submission form, place in the outer box. Note: these minimum volumes do not allow for add-on or repeat testing. We appreciate your feedback. National Library of Medicine Monday-Friday: These samples are invalid for coagulation screening tests, protein C, or factor assays. We support our products with extensive clinical expertise and educational materials to help you achieve the best possible results. Perform venipuncture using the Vacutainer needle or butterfly, Attach the tube and allow blood to fill the tube completely by vacuum, Withdraw the needle and apply direct pressure to the venipuncture site. The https:// ensures that you are connecting to the Supplier: BD 362761. However, these contaminating erythrocytes are lost after downstream procedures such as cryopreservation, and have not been observed to influence function ( Specimens for coagulation testing may also be affected by an abnormal hematocrit. Place the cells in a -80 °C freezer for 24 h. After 24 h, remove cells to a long-term LN. Cervical hematoma formation could compromise the patient’s airway. Wird es auf den mit der Punktionskanüle verbundenen Adapter gesteckt, wird durch diesen Unterdruck das Blut angesaugt. Transferring sample between tubes results in adulteration of the sample and will produce spurious test results. We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Supplier: BD 362760 Catalog No. This will be used to calculate how many aliquots of PBMCs are frozen down. B. Vacuette, Vacutainer) herrscht von vornherein ein Unterdruck. When drawing ONLY a (light) blue top tube for coagulation studies: If the venipuncture is promptly successful with a good flow of blood directly into the vacutainer, that single, filled tube may be submitted for coagulation studies. Conclusion: Results of our study confirm that the introduction of the Sarstedt S-Monovette blood tubes has reduced the hemolysis rate in the emergency department compared to the previously used BD Vacutainer® SST II Plus plastic serum tubes. The presence of clots or clot fragments in the sample is an indication of improper blood collection. Gently re-suspend the cells in 10 ml of PBS and remove an aliquot for cell counting. An example is shown below in Figure 2 from one of our own lab’s studies. All contact information provided shall also be maintained in accordance with our ZERO BIAS - scores, article reviews, protocol conditions and more Please sign in to view account pricing and product availability. Citrate tubes for coagulation tests (BLUE or LIGHT BLUE TOP), Gel (SST) tube with clot activator (GOLD TOP), Activated clot tube without gel (RED TOP), Sodium-heparin tube without gel (GREEN TOP), Lithium-heparin tube with gel (LIME GREEN TOP), Trace metal free tube w/EDTA (NAVY BLUE TOP W/ PURPLE LETTERING), Potasium-EDTA tube, plasma preparation tube (PEARL WHITE TOP). Order in which multiple samples should be drawn: Blood samples should be collected directly into vacutainer(s) in the following order to prevent cross contamination of one tube with the additive of another tube that could result in spurious lab results. The processing lab removes the PBMC from the upper compartment of the tube (above the gel plug), washes the PBMC, and can cryopreserve them using DMSO-containing media, as detailed in this protocol. Draw blood with a vacutainer needle or butterfly catheter directly into a 3.2% or 3.8% citrate tube (blue top tube) Make sure the tube is in-date and completely filled by vacuum draw. Medical professionals, medical facility employees 855.571.2100, Need help with SupplyManager?800.422.0280. Use one of the following sampling protocols: Perform venipuncture and collect blood into the syringe containing pre-measured citrate to obtain the. While the minimum volume necessary for testing is stated in the test menu, when clinically possible it is advisable to collect the preferred volume, as this will allow for repeat testing when necessary. the contents by NLM or the National Institutes of Health. Yield is easily determined by either manual or automated cell counting, and has also been reported to be roughly equivalent between CPT and manual Ficoll methods ( Medical professionals, medical facility employees 855.571.2100, Need help with SupplyManager?800.422.0280. Please see the Specimen Collection section of our Microbiology Guide for collection information, including our Microbiology Specimen Collection Guide for UCSF Operating Rooms. If blood is to be obtained from a catheter, it must be collected by nursing staff. sterile saline (0.9% saline, NO HEPARIN). Shipping Temperature. 02-688-81 / Case of 60; Due to product restrictions, please . Note: Shipping sample at 4 °C (or on wet ice), can result in platelet activation and other unwanted physiological and phenotypic changes. Saturdays (limited service): This allows the spun tubes to be shipped, maintaining the PBMC in an isolated environment from the erythrocytes and granulocytes, which may improve their viability and function. A 3 to 5 ml aliquot of blood should be withdrawn from the catheter, and then the sample syringe containing pre-measured citrate is attached to the catheter and blood is withdrawn to obtain the. DO NOT withdraw specimens from an extremity proximal to a running IV, nor from the IV line itself as this may result in contamination of the sample with the IV fluid. Sarstedt lead free li heparin sarstedt monovette vacutainer Lead Free Li Heparin Sarstedt Monovette Vacutainer, supplied by Sarstedt, used in various techniques. * In general, because of the risk of bacterial contamination, if blood cultures are needed they should always be drawn first. In BSC, gently invert the CPT tubes several times, then pipette the plasma and PBMCs into a sterile 50 ml conical tube. Samples should be redrawn for valid results. Over pipetting can result in cell rupture. If unavailable, a plastic vial with a black screw top cap is acceptable. Die Microvette findet Anwendung in der Abnahme von Kapillarblut . CPT™ is an evacuated, sterile blood . If the patient is 6 years old or more, 5 mL of blood should be drawn and discarded before the second blue top tube is filled. BD Vacutainer® SST™ Venous Blood Collection Tube Serum Tube Clot Activator / Separator Gel Additive 16 X 100 mm 8.5 mL Red / Gray Mottled Conventional Closure Plastic Tube, BD Vacutainer® Venous Blood Collection Tube Whole Blood Tube K2 EDTA Additive 13 X 75 mm 4 mL Lavender BD Hemogard™ Closure Plastic Tube, BD Vacutainer® Plus Venous Blood Collection Tube Coagulation Tube Sodium Citrate Additive 13 X 75 mm 2.7 mL Light Blue BD Hemogard™ Closure Plastic Tube, BD Vacutainer® Plus Venous Blood Collection Tube Serum Tube Clot Activator / Separator Gel Additive 13 X 100 mm 5 mL Gold BD Hemogard™ Closure Plastic Tube, Uni-Flex® Safety Cap Tube Closure Flexible Plastic Over-Locking White 13 mm For 13 mm Culture Tubes NonSterile, Uni-Flex® Safety Cap Tube Closure Flexible Plastic Over-Locking Red 13 mm For 13 mm Culture Tubes NonSterile, Uni-Flex® Safety Cap Tube Closure Flexible Plastic Over-Locking Lavender 13 mm For 13 mm Culture Tubes NonSterile, Uni-Flex® Safety Cap Tube Closure Flexible Plastic Over-Locking Green 13 mm For 13 mm Culture Tubes NonSterile, Consumers: Please visit SimplyMedical.com, McKesson Acceptable Dating: we will ship >= 90 days, Clot activator and gel for serum separation, BD Vacutainer® brand sterile hematology tubes with the BD Hemogard™ cl …, The rubber stopper is recessed inside the plastic shield so any drops …, Buffered Sodium Citrate, 0.109M (Equiv. Note: PBMC can be kept for days or even weeks at -80 °C before transfer to LN2; however, there is progressive loss of viability and function with longer storage at -80 °C. Privacy Policy. BD Vacutainer Tubes are used to transport. Ruitenberg et al., 2006 Make sure the tube is in-date and completely filled by vacuum draw. Add 1 ml of the cell suspension to each pre-chilled cryovial and place the vials in a CoolCell or Mr. Frosty. Fisher Scientific is always working to improve our content for you. Place the appropriate number of cryovials on ice or in a cooling rack in the 4 °C to begin chilling the vials in preparation for freezing. The BD Vacutainer® Blood Collection System is a closed evacuated system, which consists of a sterile double-ended needle with safety valve, BD Vacutainer® One-Use holder and a sterile BD Vacutainer® Evacuated Blood Collection Tube with predetermined draw. P10 and P200 mechanical pipettors (any vendor), 50 ml Falcon (or similar) conical polypropylene tubes, Foam packing material and shipping containers, A centrifuge capable of reaching speeds of 1,800, 50 ml conical adapters and buckets for centrifuge, Automated cell counter or microscope and hemacytometer (any vendor), Biosafety cabinet level A2 (BSC) (any vendor), -80 °C freezer (for initial cryopreservation) (any vendor). Counts on multiple tubes are rarely required unless Tube #3 is visibly bloody at which point a cell count on Tube #1 may be requested. DO NOT Transfer samples between vacutainer types: Never transfer blood from one tube type to another to make up for short volume. Cellular Preparation Tubes (CPTs) are Vacutainer blood draw tubes that contain Ficoll-Hypaque and a gel plug that separates the Ficoll solution from the blood to be drawn. Email: diagcenter@cornell.edu, Business Hours The tubes are stocked in the Memory & Aging clinic and are available from laboratory processing areas at each hospital. Label the sample tube with patient identifiers. Material: Plastic: Sterility: Sterile: For Use With (Application) For UA chemistry Urine testing: Storage Requirements: Room temperature: View More Specs. Aspirate the supernatant carefully to not disturb cell pellet. Siliconized glass tubes can be used as centrifugation and storage tubes, however glass tubes may crack, or shatter. 8:00am-5:00pm You may enter multiple email addresses separated by semicolons. 203 20K views 2 years ago Blutentnahme mit der SARSTEDT S-Monovette® im EU-Farbcode. Loveland, CO 80538. Because erythrocytes and granulocytes are much more fragile to freezing and thawing, PBMC isolation is a common prerequisite to cryopreservation of blood cells; and the most common method for PBMC isolation is density gradient centrifugation using Ficoll-Hypaque, a high molecular weight carbohydrate solution. To some degree, the latter can be determined visually, as erythrocyte contamination will redden the pellet of PBMC acquired after centrifugation. Note: Human AB Serum must be heat inactivated prior to use. application, vein puncture, blood draw, correct needle disposal to the appropriate sample storage. Corkum et al., 2015 Hemolyzed plasma is an indication of improper sampling, unless the patient has a hemolytic disease process. Accessibility BD Vacutainer™ Urine Collection Kits: Urinalysis Reduce the need to retest, relabel, and/or recollect specimens $64.40 - $130.60 Specifications. If a patient's hematocrit is above 55%, contact the Hematology laboratory and request a tube adjusted to contain the appropriately decreased amount of citrate anticoagulant. The BD Vacutainer™ Eclipse™ Blood Collection Needle features a safety shield that allows for one-handed activation to cover the needle immediately upon withdrawal from the vein and confirms proper activation with an audible click. Additive: K2EDTA (spray dried), 7.2mg. Franklin Lakes, NJ 07417-1885 The sample should be redrawn if the Vacutainer tube or syringe is not filled to the desired sample volume. BD Vacutainer® SST™ Venous Blood Collection Tube Serum Tube Clot Activator / Separator Gel Additive 16 X 100 mm 8.5 mL Red / Gray Mottled Conventional Closure Plastic Tube, BD Vacutainer® Venous Blood Collection Tube Whole Blood Tube K2 EDTA Additive 13 X 75 mm 4 mL Lavender BD Hemogard™ Closure Plastic Tube, BD Vacutainer® Plus Venous Blood Collection Tube Coagulation Tube Sodium Citrate Additive 13 X 75 mm 2.7 mL Light Blue BD Hemogard™ Closure Plastic Tube, BD Vacutainer® Plus Venous Blood Collection Tube Serum Tube Clot Activator / Separator Gel Additive 13 X 100 mm 5 mL Gold BD Hemogard™ Closure Plastic Tube, Uni-Flex® Safety Cap Tube Closure Flexible Plastic Over-Locking White 13 mm For 13 mm Culture Tubes NonSterile, Uni-Flex® Safety Cap Tube Closure Flexible Plastic Over-Locking Red 13 mm For 13 mm Culture Tubes NonSterile, Uni-Flex® Safety Cap Tube Closure Flexible Plastic Over-Locking Lavender 13 mm For 13 mm Culture Tubes NonSterile, Uni-Flex® Safety Cap Tube Closure Flexible Plastic Over-Locking Green 13 mm For 13 mm Culture Tubes NonSterile, Consumers: Please visit SimplyMedical.com, McKesson Acceptable Dating: we will ship >= 30 days, Sodium Heparin (spray-coated), 68 USP units, Clot activator and gel for serum separation, BD Vacutainer® brand sterile hematology tubes with the BD Hemogard™ cl …, The rubber stopper is recessed inside the plastic shield so any drops …, Buffered Sodium Citrate, 0.109M (Equiv. We appreciate your feedback. BD Vacutainer® CPT™ is a fully-closed system for separation of mononuclear cells from whole blood, where cell separation is carried out in the primary blood collection tube. Urine containers requiring special preservatives are not stocked on the floors, but can be obtained from the specimen processing desk at each hospital laboratory. Keywords: blood specimen collection; catheter; emergency department; hemolysis; preanalytical phase. The rubber stopper is recessed inside the plastic shield so any drops …. Given the frequent need to conserve blood volume in our pediatric patient population, please see our list of Pediatric Minimum Whole Blood Draw Volumes for the minimum sample volumes needed for certain frequently ordered tests. Samples drawn into EDTA, heparin, or other anticoagulants or samples drawn into clot activator or serum separator tubes are invalid for coagulation testing. Blood samples can be drawn from an indwelling catheter using the syringe method, if the catheter is first flushed with 5 to 10 ml. Transfer citrated blood sample to a plastic or no-additive glass tube for centrifugation. 2915 Rocky Mountain Avenue, Suite 400. Vacutainer tubes may contain additives designed to stabilize and preserve the specimen prior to analytical testing. Please follow this link for some useful phlebotomy/specimen collection policies and procedures. Furthermore, when used with studies or sites which ship blood samples overnight, PBMCs from CPTs have a higher purity and less infiltrate from other (contaminating) cell types, such as red blood cells, in contrast to samples shipped in standard blood collection tubes over a 24-48 h period ( R IN BD CLINICAL ADVANTAGE: IT RBD Vacutainer®PST™Lithium Heparin Tubes eliminate the need to wait for a clot to form, making it an ideal tube for STAT procedures, as well as for patients T receiving anticoagulant therapy. Each vial should be labeled with patient information (full name and medical record number are minimum requirements) and also list the name of the person who collected the specimen and the date it was collected. Complete filling of vacutainers (especially for coagulation testing): Liquid-containing vacutainers (e.g. There is no standard at present for adjusting the amount of anticoagulant for specimens with low hematocrits. Ruitenberg et al., 2006 Gently restrain the patient and apply manual pressure proximal to cephalic or saphenous venipuncture site to allow the vein to fill with blood. Atraumatic venipuncture technique is critical to prevent activation/depletion of clotting factors during collection. Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, Palo Alto, CA, USA. Draw an exact volume of citrate into a syringe to obtain 1 of the following total samples: Perform venipuncture to collect total sample volume. Your feedback has been submitted. An official website of the United States government. Please sign in to view account pricing and product availability. Darüber hinaus gibt es noch die sogenannte V-Monovette, die zur Abnahme von Harn verwendet wird. Aspirate the supernatant from the cells and re-suspend them with the volume of ‘A’ calculated in step 5c. Also, repeated cycling between -80 °C (or dry ice) and LN2 will negatively affect viability and function. Blood collection with the SARSTEDT S-Monovette® (ISO colour code). Refrigerate plasma for same-day shipment or store frozen for up to 2 weeks. Compare #207068; 1,366; For at home specimen collections, appropriate specimen containers and patient instructions can be obtained from any of our outpatient specimen collection facilities. This information is also available via the individual test entries in our test menu. The ratio of citrate to blood is critical for valid results. Provides fast, easy needle-tip protection. For the one-step isolation of lymphocytes, monocytes, and plasma. Check the expiration date of all tubes prior to collecting samples and discard any expired tubes. Note: It is important to be efficient through this process, as warming DMSO is toxic to the PBMCs. Aspirate an exact volume of citrate into the collection syringe to give a final ratio of 1 part citrate to 9 parts blood. Die sogenannte S-Monovette wird mit entsprechenden Zusätzen zur Serum -Blutentnahme verwendet. This item has been discontinued and is no longer available. Part ‘A’ is made up of 100% Human AB Serum and Part ‘B’ is made up of 80% Human AB Serum and 20% DMSO. Companion Animal Hospital in Ithaca, NY for cats, dogs, exotics, and wildlife, Equine and Nemo Farm Animal Hospitals in Ithaca, NY for horses and farm animals, Cornell Ruffian Equine Specialists, on Long Island for every horse, Ambulatory and Production Medicine for service on farms within 30 miles of Ithaca, NY, Animal Health Diagnostic Center New York State Veterinary Diagnostic Laboratory, Cornell University College of Veterinary Medicine Ithaca, New York 14853-6401, Business Hours - Monday-Friday: 8:00am to 5:00pm; Saturdays: 9:00am-1:00pm. Phone: (607) 253-3900 Note: CSF samples for Beta Amyloid 42/Tau Protein Analysis must be collected in a special polypropylene tube to prevent adherence of the protein to the sides of the tube. Isolation and cryopreservation of peripheral blood mononuclear cells (PBMC) is common practice in clinical studies that employ cellular immune assays. Stichworte: Aspirationsprinzip, Blutentnahme, Unterdruckprinzip. Corkum C., Ings D., Burgess C., Karwowska S., Kroll W. and Michalak T.(2015). Whereas in the first eight weeks, all specimens were collected using Vacutainer®, in the second eight weeks, blood was taken using S-Monovette® in aspiration mode. ). A Pasteur pipette and vacuum can be used for aspirating as well as manually aspirating with serological pipettes and a pipette gun. to 3.2% Sodium Citrate), BD Vacutainer® brand sterile chemistry tubes with the BD Hemogard™ clo …, The rubber stopper is recessed inside the plastic shield, so any drops …, The Uni-Flex® Safety Cap liquid tight seal ensures sample integrity an …, Uni-Flex® Safety Caps protect your laboratory staff from the aerosols …, The Uni-Flex® Safety Cap is designed to hold under centrifugation, agi …. Divide your total number of aliquots in half to determine what volume of each media you will need. Centrifuge the whole blood sample for 10 to 15 minutes. BD #367861. Consistency of centrifugation step. View more versions of this product. 3. This decreases the complexity of steps for mononuclear cell separation, thereby minimizing variability from sample processing. Only samples in good condition (no visible clumping or erythrocyte contamination) with > 20-cc blood volume were included for comparison. Specifications. sharing sensitive information, make sure you’re on a federal Your samples will be frozen in a 50:50 mixture of Part ‘A’ and Part ‘B’, totaling a final concentration of 10% DMSO. to 3.2% Sodium Citrate), BD Vacutainer® brand sterile chemistry tubes with the BD Hemogard™ clo …, The rubber stopper is recessed inside the plastic shield, so any drops …. Cryopreservation allows for batching of samples, which is convenient and improves the comparability of data. BD Vacutainer® Venous Blood Collection Tube Whole Blood Tube K3 EDTA Additive 13 X 100 mm 7 mL Lavender Conventional Closure Glass Tube TUBE, BLD COL K3EDTA LAV 7ML GLS VACUTAINER (100/BX 10BX/CS) Compare Features K3 EDTA 15% solution, 0.081 ml, 12.15 mg More … Questions? Electrophoresis, Western Blotting and ELISA, Chromatography and Mass Spectrometry Reagents, Laboratory Syringe Needles and Accessories, Lab Coats, Aprons, and Other Safety Apparel, Sharps Disposal Containers and Accessories, Classroom Laboratory Supplies and Consumables, Applied Biosystems™ TaqMan® Assay and Arrays Search Tool, Applied Biosystems™ TaqMan® Custom Assay Design Tools, Applied Biosystems™ Custom qPCR Primers and TaqMan® Probes Tool, Chemical Storage and Management Resource Center, For the separation of mononuclear cells from whole blood, During centrifugation, a gel barrier in the tube isolates the mononuclear cells and plasma from the erythrocytes and granulocytes, May also be used to obtain highly concentrated mononuclear cells and/or plasma for immunotyping, culture, and cell function studies, and for tests using gene-amplification techniques, Mononuclear cells may be resuspended in plasma to retain viability for storage and transportation. We will not share your information for any other purposes. and transmitted securely. Because of the different maximum draw volumes, there is a wider range of blood volumes for the SepMate study; but recovered cells/ml were very similar with CPT and SepMate tubes. If this is not done, spurious results are likely to be obtained, e.g., an elevated PTT from residual heparin or a spuriously elevated glucose or potassium from the remnants of an intravenous solution. Quality results begin with proper specimen collection! Complete filling of vacutainers (especially for coagulation testing): 4. This tutorial video describes the blood collection with aspiration technique and the safety-needle.The blood collection process is explained step-by-step, from the required material, tourniquet incl. Each aliquot will have a total of 1 ml of freezing media. The integrated safety shield and holder maximize OSHA compliance by protecting clinicians from potential front and back-end needlestick injuries. Ruitenberg et al., 2006 100. If blood is obtained from an intravascular line, it is important to clear the line of the fluid which has been infused through it or is "keeping it open" (e.g. 6. The BD Vacutainer™ Eclipse™ Blood Collection Needle features a safety shield that allows for one-handed activation to cover the needle immediately upon withdrawal from the vein and confirms proper activation with an audible click. Show more Show more Sodium citrate (3.2 or 3.8%) is the only acceptable anticoagulant for coagulation assays. Note that if a winged blood collection set ("butterfly") is used and the coagulation tube is the first tube drawn, a discard blue top tube should be used to clear the dead-space volume of the line or the line should be allowed to 'fill' prior to attaching the vacutainer adapter. GTIN - Shelfpack. Use of peripheral veins, rather than the jugular vein, is preferred for patients having severe hemostatic defects because of the risk of hematoma formation at venipuncture sites. Privacy Policy. Schlenke P., Klüter H., Müller-Steinhardt M., Hammers H. J., Borchert K. and Bein G.(1998). All contact information provided shall also be maintained in accordance with our Diagnostic Use 363047 363048 INTENDED USE BD Vacutainer Tubes, Needles and Holders are used together as a system for the collection of venous blood. Cellular Preparation Tubes (CPTs), which contain Ficoll-Hypaque, simplify the standard procedure for density gradient centrifugation in two ways: (1) blood is collected into the same tube that is then used to isolate the PBMC; and (2) the tube is pre-loaded with Ficoll-Hypaque, which is separated by a gel plug so that it is not disturbed by the entry of blood into the tube. Peripheral blood mononuclear cell (PBMC) isolation is commonly done via density gradient centrifugation over Ficoll-Hypaque, a labor-intensive procedure that requires skilled technicians and can contribute to sample variability.